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RNA Name

Base Composition Base CompositionUridinePseudouridineN1-Methyl-Pseudouridine

Template Plasmid Source Template Plasmid SourceExothera suppliedCustomer supplied

Please upload or paste your ORF sequence including start codon and the stop codon

DNA Length

RNA Length

Template Type Template Type Circular plasmid Linear plasmid PCR-product transcription template

Please confirm that the DNA is compatible with co-transcriptional capping and contains the following elements: 1. T7 promoter, 2. First two nt after the T7 promoter are AG, 3. 5’ UTR, 4. CDS, 5. 3’ UTR, 6. Poly(A) tail of 80 to 120 nt, 7. Unique BspQI (SapI) linearization site cutting at the end of the poily(A) tail (no residual nt after poly(A) tail) Please confirm that the DNA is compatible with co-transcriptional capping and contains the following elements: 1. T7 promoter, 2. First two nt after the T7 promoter are AG, 3. 5’ UTR, 4. CDS, 5. 3’ UTR, 6. Poly(A) tail of 80 to 120 nt, 7. Unique BspQI (SapI) linearization site cutting at the end of the poily(A) tail (no residual nt after poly(A) tail) Yes No

Does your template have a polyA track? Does your template have a polyA track? Yes No

Target Yield Target Yield200 µg1 mg5 mg10 mg25 mg50 mgOther (specify)

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